show Abstracthide AbstractThis is a de novo assembly of Bos taurus using long reads for assembly and short reads for scaffolding and polishing. The assembly was scaffolded using a combination of in vitro chromosome conformation capture sequencing, optical maps, and a recombination map. Subsequent scaffolding was confirmed using genetic linkage maps and a Radiation Hybrid map. This material is based on work supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, National Research Support Project NRSP8 (Cattle Genome Coordination)